What is RT- PCR?

Reverse transcription polymerase chain reaction (RT-PCR) is a variation of standard PCR that involves the amplification of specific mRNA obtained from small samples. It eliminates the need for the tedious mRNA purification process required for conventional cloning techniques. In RT-PCR, reverse transcriptase and an RNA sample are used in addition to the standard PCR reagents. The reaction mixture is heated to 37 ˚C, which enables the production of cDNA from the RNA sample by reverse transcription. This cDNA anneals to one of the primers leading to first-strand synthesis. Standard PCR proceeds and dsDNA is produced.

The primers used must match the project. If amplification of all of a cell’s mRNA is required, oligo (dT) primers are sufficient because they anneal to the poly(A) tails. If a specific mRNA is to be amplified, a coding region-specific primer can be used.

RT-PCR is a common virology diagnostic method and is frequently combined with quantitative real-time PCR (qPCR), which is widely used to quantify RNA transcript levels in cells and tissues.1 The combination of real-time PCR (qPCR) and reverse transcription PCR is known as quantitative RT-PCR or qRT-PCR

What is Multiplex PCR?

Multiplex polymerase chain reaction refers to the use of polymerase chain reaction to amplify several different DNA sequences simultaneously. This process amplifies DNA in samples using multiple primers and a temperature-mediated DNA polymerase in a thermal cycler.

Meril COVID-19 one Step RT-PCR Kit

Due to the novel coronavirus (SARS-CoV-2) outbreak, Meril Diagnostics has worked extensively to develop a new one step multiplex real-time RT-PCR kit to allow laboratories to diagnose COVID-19 caused by SARS-CoV-2 infection with fast and easy to use assays. Meril Covid-19 one step Rt PCR kit is a fast, highly sensitive multiplex diagnostic kit that contains both the assays and controls needed for the real-time PCR detection of RNA from the SARS-CoV-2 virus.

It is a One tube Multiplex PCR reaction for identification and detection of 2019-ncov.  Its Primer and Probe mix based on dual-target gene design the two confirmatory gene ORF 1ab gene and nucleoprotein N gene for the detection of Sars COV 2 in human sample, with an analytical Analytical Accuracy of < 5 RNA copies/ reaction it makes Meril COVID-19 one Step RT_PCR Kit a highly sensitive and specific kit for the detection of SARS COV 2 RNA.

The kit is highly compatible with Open ended RT PCR instruments with FAM, HEX/VIC, RED/ROX channels. The easy protocol, minimum assay time and high specificity and sensitive makes it an ideal choice for your laboratory for the detection of COVID 19 suspected patients.

Meril COVID-19 RT-PCR Kit compatible with popular Applied Biosystems (ABI) 7500, BioRad CFX 96, Shanghai Hongshi SLAN-96P, QIAGEN Rotor Gene Q  and other PCR instruments with FAM, HEX/VIC, RED/ROX channels.


The primer and probe mix for this kit adopts the dual-target gene design, which targets the specific conserved sequence encoding the ORF 1ab gene and the nucleoprotein N gene. With the PCR reaction mix provided, the amplification of template can be quantitatively monitored by the increasing fluorescence signal detected by a real time PCR instrument.

The PCR detection system includes an endogenous internal control primer and probe mix. The result of internal control provides the accuracy of sampling and extraction process, in order to avoid false negative results.

Intended Use:

This kit is designed to detect COVID-19 disease using real time PCR. The results can be used to assist diagnosis of patients with COVID-19 infection, and provide molecular diagnostic basis for infected patients.

This RT-PCR kit can be used by clinical and public health laboratories to quickly evaluate up to 94 patient specimens in under 3 hours with one kit. The kit is approved for use with RNA extracted from nasopharyngeal swabs, nasopharyngeal aspirate (nasal aspirate), and bronchoalveolar lavage (BAL) from patients at risk of exposure to the SARS-CoV-2 virus or with signs and symptoms of COVID-19.

Results are for the identification of SARS-CoV-2 RNA. The SARS-CoV-2 RNA is generally detectable in nasopharyngeal swab, nasopharyngeal aspirate, and bronchoalveolar lavage (BAL) specimens during the acute phase of infection. Positive results are indicative of the presence of SARS-CoV-2 RNA; clinical correlation with patient history and other diagnostic information is necessary to determine patient infection status. Positive results do not rule out bacterial infection or co-infection with other viruses. The agent detected may not be the definite cause of disease.
Negative results do not preclude SARS-CoV-2 infection and should not be used as the sole basis for patient management decisions. Negative results must be combined with clinical observations, patient history, and epidemiological information.

Testing with the Meril COVID-19 Onse Step RT PCR  Kit is intended for use by qualified and trained clinical laboratory personnel specifically instructed and trained in the techniques of real-time PCR and in vitro diagnostic procedures.

The test results of this kit are for clinical reference only and should not be used as the only standard for clinical diagnosis. It is recommended to conduct a comprehensive analysis by combining the test results with patients’ symptoms and other laboratory tests.



Click here to know more about Covid-19 testing solutions

  • One tube reaction for identification and detection of 2019-ncov
  • Multiplex PCR
  • Primer and Probe mix based on dual-target gene design
  • Target gene ORF 1ab gene and nucleoprotein N gene
  • Analytical Accuracy of < 5 RNA copies/ reaction



  • Early detection of the novel coronavirus (SARS COV-2) nucleic acids (RNA) in patient samples

Product Specifications

Intended Use One-step RT-PCR Kit is a Real-Time Detection kit based on Multiplex PCR technology that identifies and detects the novel coronavirus (SARS COV-2) nucleic acids (RNA) in human serum nasopharyngeal swabs and throat swab samples
Principle RT-PCR
Technique Real time RT-PCR
Detection Presence of SARS COV-2 nucleic acids (RNA)
Target Gene Orf 1ab gene, Nucleoprotein N gene
Type of Specimen required Human serum, Nasopharyngeal swabs, Throat swab samples, Sputum
Assay Time 65 mins
Limit of detection <5 RNA Copies/Reaction
Diagnostic Sensitivity High
Diagnostic Specificity High
Evaluations ICMR (NIV)

Size Chart

Ordering Information

Product Code Material Description Pack Size
NCVPCR-02 Meril COVID-19 One-step RT-PCR Kit 96 Tests

Clinical Data